Generation of live attenuated novel influenza virus A/California/7/09 (H1N1) vaccines with high yield in embryonated chicken eggs.
Identifieur interne : 000E81 ( Main/Exploration ); précédent : 000E80; suivant : 000E82Generation of live attenuated novel influenza virus A/California/7/09 (H1N1) vaccines with high yield in embryonated chicken eggs.
Auteurs : Zhongying Chen [États-Unis] ; Weijia Wang ; Helen Zhou ; Amorsolo L. Suguitan ; Cindy Shambaugh ; Lomi Kim ; Jackie Zhao ; George Kemble ; Hong JinSource :
- Journal of virology [ 1098-5514 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Chiens, Embryon de poulet (virologie), Humains, Hémagglutinines (génétique), Lignée cellulaire, Mutagenèse dirigée, Sialidase (génétique), Sous-type H1N1 du virus de la grippe A (croissance et développement), Sous-type H1N1 du virus de la grippe A (génétique), Sous-type H1N1 du virus de la grippe A (immunologie), Substitution d'acide aminé, Vaccins atténués.
- MESH :
- croissance et développement : Sous-type H1N1 du virus de la grippe A.
- génétique : Hémagglutinines, Sialidase, Sous-type H1N1 du virus de la grippe A.
- immunologie : Sous-type H1N1 du virus de la grippe A.
- virologie : Embryon de poulet.
- Animaux, Chiens, Humains, Lignée cellulaire, Mutagenèse dirigée, Substitution d'acide aminé, Vaccins atténués.
English descriptors
- KwdEn :
- Amino Acid Substitution, Animals, Cell Line, Chick Embryo (virology), Dogs, Hemagglutinins (genetics), Humans, Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (growth & development), Influenza A Virus, H1N1 Subtype (immunology), Mutagenesis, Site-Directed, Neuraminidase (genetics), Vaccines, Attenuated.
- MESH :
- chemical , genetics : Hemagglutinins, Neuraminidase.
- genetics : Influenza A Virus, H1N1 Subtype.
- growth & development : Influenza A Virus, H1N1 Subtype.
- immunology : Influenza A Virus, H1N1 Subtype.
- virology : Chick Embryo.
- Amino Acid Substitution, Animals, Cell Line, Dogs, Humans, Mutagenesis, Site-Directed, Vaccines, Attenuated.
Abstract
Several live attenuated influenza virus A/California/7/09 (H1N1) (CA09) candidate vaccine variants that possess the hemagglutinin (HA) and neuraminidase (NA) gene segments from the CA09 virus and six internal protein gene segments from the cold-adapted influenza virus A/Ann Arbor/6/60 (H2N2) virus were generated by reverse genetics. The reassortant viruses replicated relatively poorly in embryonated chicken eggs. To improve virus growth in eggs, reassortants expressing the HA and NA of CA09 were passaged in MDCK cells and variants exhibiting large-plaque morphology were isolated. These variants replicated at levels approximately 10-fold higher than the rate of replication of the parental strains in embryonated chicken eggs. Sequence analysis indicated that single amino acid changes at positions 119, 153, 154, and 186 were responsible for the improved growth properties in MDCK cells and eggs. In addition, the introduction of a mutation at residue 155 that was previously shown to enhance the replication of a 1976 swine influenza virus also significantly improved the replication of the CA09 virus in eggs. Each variant was further evaluated for receptor binding preference, antigenicity, attenuation phenotype, and immunogenicity. Mutations at residues 153, 154, and 155 drastically reduced viral antigenicity, which made these mutants unsuitable as vaccine candidates. However, changes at residues 119 and 186 did not affect virus antigenicity or immunogenicity, justifying their inclusion in live attenuated vaccine candidates to protect against the currently circulating 2009 swine origin H1N1 viruses.
DOI: 10.1128/JVI.02106-09
PubMed: 19864389
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000219
- to stream PubMed, to step Curation: 000219
- to stream PubMed, to step Checkpoint: 000186
- to stream Ncbi, to step Merge: 000432
- to stream Ncbi, to step Curation: 000432
- to stream Ncbi, to step Checkpoint: 000432
- to stream Main, to step Merge: 000E87
- to stream Main, to step Curation: 000E81
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Generation of live attenuated novel influenza virus A/California/7/09 (H1N1) vaccines with high yield in embryonated chicken eggs.</title><author><name sortKey="Chen, Zhongying" sort="Chen, Zhongying" uniqKey="Chen Z" first="Zhongying" last="Chen">Zhongying Chen</name><affiliation wicri:level="1"><nlm:affiliation>MedImmune, Mountain View, CA 94043, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>MedImmune, Mountain View, CA 94043</wicri:regionArea><wicri:noRegion>CA 94043</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Weijia" sort="Wang, Weijia" uniqKey="Wang W" first="Weijia" last="Wang">Weijia Wang</name></author><author><name sortKey="Zhou, Helen" sort="Zhou, Helen" uniqKey="Zhou H" first="Helen" last="Zhou">Helen Zhou</name></author><author><name sortKey="Suguitan, Amorsolo L" sort="Suguitan, Amorsolo L" uniqKey="Suguitan A" first="Amorsolo L" last="Suguitan">Amorsolo L. Suguitan</name></author><author><name sortKey="Shambaugh, Cindy" sort="Shambaugh, Cindy" uniqKey="Shambaugh C" first="Cindy" last="Shambaugh">Cindy Shambaugh</name></author><author><name sortKey="Kim, Lomi" sort="Kim, Lomi" uniqKey="Kim L" first="Lomi" last="Kim">Lomi Kim</name></author><author><name sortKey="Zhao, Jackie" sort="Zhao, Jackie" uniqKey="Zhao J" first="Jackie" last="Zhao">Jackie Zhao</name></author><author><name sortKey="Kemble, George" sort="Kemble, George" uniqKey="Kemble G" first="George" last="Kemble">George Kemble</name></author><author><name sortKey="Jin, Hong" sort="Jin, Hong" uniqKey="Jin H" first="Hong" last="Jin">Hong Jin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:19864389</idno><idno type="pmid">19864389</idno><idno type="doi">10.1128/JVI.02106-09</idno><idno type="wicri:Area/PubMed/Corpus">000219</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000219</idno><idno type="wicri:Area/PubMed/Curation">000219</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000219</idno><idno type="wicri:Area/PubMed/Checkpoint">000186</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000186</idno><idno type="wicri:Area/Ncbi/Merge">000432</idno><idno type="wicri:Area/Ncbi/Curation">000432</idno><idno type="wicri:Area/Ncbi/Checkpoint">000432</idno><idno type="wicri:Area/Main/Merge">000E87</idno><idno type="wicri:Area/Main/Curation">000E81</idno><idno type="wicri:Area/Main/Exploration">000E81</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Generation of live attenuated novel influenza virus A/California/7/09 (H1N1) vaccines with high yield in embryonated chicken eggs.</title><author><name sortKey="Chen, Zhongying" sort="Chen, Zhongying" uniqKey="Chen Z" first="Zhongying" last="Chen">Zhongying Chen</name><affiliation wicri:level="1"><nlm:affiliation>MedImmune, Mountain View, CA 94043, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>MedImmune, Mountain View, CA 94043</wicri:regionArea><wicri:noRegion>CA 94043</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Weijia" sort="Wang, Weijia" uniqKey="Wang W" first="Weijia" last="Wang">Weijia Wang</name></author><author><name sortKey="Zhou, Helen" sort="Zhou, Helen" uniqKey="Zhou H" first="Helen" last="Zhou">Helen Zhou</name></author><author><name sortKey="Suguitan, Amorsolo L" sort="Suguitan, Amorsolo L" uniqKey="Suguitan A" first="Amorsolo L" last="Suguitan">Amorsolo L. Suguitan</name></author><author><name sortKey="Shambaugh, Cindy" sort="Shambaugh, Cindy" uniqKey="Shambaugh C" first="Cindy" last="Shambaugh">Cindy Shambaugh</name></author><author><name sortKey="Kim, Lomi" sort="Kim, Lomi" uniqKey="Kim L" first="Lomi" last="Kim">Lomi Kim</name></author><author><name sortKey="Zhao, Jackie" sort="Zhao, Jackie" uniqKey="Zhao J" first="Jackie" last="Zhao">Jackie Zhao</name></author><author><name sortKey="Kemble, George" sort="Kemble, George" uniqKey="Kemble G" first="George" last="Kemble">George Kemble</name></author><author><name sortKey="Jin, Hong" sort="Jin, Hong" uniqKey="Jin H" first="Hong" last="Jin">Hong Jin</name></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Substitution</term><term>Animals</term><term>Cell Line</term><term>Chick Embryo (virology)</term><term>Dogs</term><term>Hemagglutinins (genetics)</term><term>Humans</term><term>Influenza A Virus, H1N1 Subtype (genetics)</term><term>Influenza A Virus, H1N1 Subtype (growth & development)</term><term>Influenza A Virus, H1N1 Subtype (immunology)</term><term>Mutagenesis, Site-Directed</term><term>Neuraminidase (genetics)</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Chiens</term><term>Embryon de poulet (virologie)</term><term>Humains</term><term>Hémagglutinines (génétique)</term><term>Lignée cellulaire</term><term>Mutagenèse dirigée</term><term>Sialidase (génétique)</term><term>Sous-type H1N1 du virus de la grippe A (croissance et développement)</term><term>Sous-type H1N1 du virus de la grippe A (génétique)</term><term>Sous-type H1N1 du virus de la grippe A (immunologie)</term><term>Substitution d'acide aminé</term><term>Vaccins atténués</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hemagglutinins</term><term>Neuraminidase</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Hémagglutinines</term><term>Sialidase</term><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Embryon de poulet</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chick Embryo</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Substitution</term><term>Animals</term><term>Cell Line</term><term>Dogs</term><term>Humans</term><term>Mutagenesis, Site-Directed</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chiens</term><term>Humains</term><term>Lignée cellulaire</term><term>Mutagenèse dirigée</term><term>Substitution d'acide aminé</term><term>Vaccins atténués</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Several live attenuated influenza virus A/California/7/09 (H1N1) (CA09) candidate vaccine variants that possess the hemagglutinin (HA) and neuraminidase (NA) gene segments from the CA09 virus and six internal protein gene segments from the cold-adapted influenza virus A/Ann Arbor/6/60 (H2N2) virus were generated by reverse genetics. The reassortant viruses replicated relatively poorly in embryonated chicken eggs. To improve virus growth in eggs, reassortants expressing the HA and NA of CA09 were passaged in MDCK cells and variants exhibiting large-plaque morphology were isolated. These variants replicated at levels approximately 10-fold higher than the rate of replication of the parental strains in embryonated chicken eggs. Sequence analysis indicated that single amino acid changes at positions 119, 153, 154, and 186 were responsible for the improved growth properties in MDCK cells and eggs. In addition, the introduction of a mutation at residue 155 that was previously shown to enhance the replication of a 1976 swine influenza virus also significantly improved the replication of the CA09 virus in eggs. Each variant was further evaluated for receptor binding preference, antigenicity, attenuation phenotype, and immunogenicity. Mutations at residues 153, 154, and 155 drastically reduced viral antigenicity, which made these mutants unsuitable as vaccine candidates. However, changes at residues 119 and 186 did not affect virus antigenicity or immunogenicity, justifying their inclusion in live attenuated vaccine candidates to protect against the currently circulating 2009 swine origin H1N1 viruses.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Jin, Hong" sort="Jin, Hong" uniqKey="Jin H" first="Hong" last="Jin">Hong Jin</name><name sortKey="Kemble, George" sort="Kemble, George" uniqKey="Kemble G" first="George" last="Kemble">George Kemble</name><name sortKey="Kim, Lomi" sort="Kim, Lomi" uniqKey="Kim L" first="Lomi" last="Kim">Lomi Kim</name><name sortKey="Shambaugh, Cindy" sort="Shambaugh, Cindy" uniqKey="Shambaugh C" first="Cindy" last="Shambaugh">Cindy Shambaugh</name><name sortKey="Suguitan, Amorsolo L" sort="Suguitan, Amorsolo L" uniqKey="Suguitan A" first="Amorsolo L" last="Suguitan">Amorsolo L. Suguitan</name><name sortKey="Wang, Weijia" sort="Wang, Weijia" uniqKey="Wang W" first="Weijia" last="Wang">Weijia Wang</name><name sortKey="Zhao, Jackie" sort="Zhao, Jackie" uniqKey="Zhao J" first="Jackie" last="Zhao">Jackie Zhao</name><name sortKey="Zhou, Helen" sort="Zhou, Helen" uniqKey="Zhou H" first="Helen" last="Zhou">Helen Zhou</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Chen, Zhongying" sort="Chen, Zhongying" uniqKey="Chen Z" first="Zhongying" last="Chen">Zhongying Chen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E81 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E81 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:19864389
|texte= Generation of live attenuated novel influenza virus A/California/7/09 (H1N1) vaccines with high yield in embryonated chicken eggs.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19864389" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |